A microfluidic device for:
1. Encapsulation of subsets of microbiota - Multiple droplet generation by slanted T-junction geometry
2. Cultivation of microbial subsets - Monitoring of cell growth by microscopy
3. Analysis of cultivated communities
using monodispersed droplets in oil phase. The technology is being applied to cultivation of tunicate microbiota to isolate E.frumentensis, an anti-cancer drug producing bacterium.